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HSP110 Antibody

  • IHC - HSP110 Antibody ASM10456
    Immunohistochemistry analysis using Rabbit Anti-HSP110 Polyclonal Antibody (ASM10456). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (ASM10456) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Positive epidermal, dermal and muscle staining.
  • WB - HSP110 Antibody ASM10456
    Western blot analysis of Human Cell line lysates showing detection of HSP110 protein using Rabbit Anti-HSP110 Polyclonal Antibody (ASM10456). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP110 Polyclonal Antibody (ASM10456) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92599
Other Accession NP_006635.3
Host Rabbit
Reactivity Human, Mouse, Rat, Hamster, Monkey, Bovine, Yeast, Sheep
Clonality Polyclonal
Description Rabbit Anti-Hamster HSP110 Polyclonal
Target/Specificity Detects ~110kDa.
Other Names Heat shock 105kDa alpha Antibody, heat shock 105kDa beta Antibody, HSP105 Antibody, HSP105A Antibody, HSP105B Antibody, HSP110 Antibody, HSPH1 Antibody, NY CO 25 Antibody
Immunogen Synthetic peptide derived from the sequence of hamster HSP110; sequence identical to human and mouse
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.2, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-195 was sufficient for detection of HSP110 in 10 µg of human cell line mixed lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm
Research Areas
Citations (0)

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HSP110 belongs to a family of large stress proteins known as the HSP110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied HSP70 family. They share 30-33% amino acid identity, mostly in the conserved ATP-binding domain (1). HSP110 cooperates with HSP70 in protein folding in the eukaryotic cytosol (2). In mammals, HSP110 is constitutively expressed, but exhibits particularly high levels in the brain. Both HSP70 and HSP110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of HSP110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of HSP70 (3). Studies also suggest that HSP110 can be used in heat shock protein-based cancer immunotherapy (4).


1. Lee-Yoon D., et al. (1995) J Biol Chem. 270: 15725-15733.
2. Dragovic Z., et al. (2006) EMBO J. 25: 2519-2528.
3. Nakamura J., et al. (2008) Stroke. 39(10): 2853-2859.
4. Subject D.R. (2001) Health Research Inc. Report number AS90893: 1-84.

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Cat# ASM10456
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