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Piccolo Antibody

  • IHC - Piccolo Antibody ASM10460
    Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody (ASM10460). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (ASM10460) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.
  • WB - Piccolo Antibody ASM10460
    Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody (ASM10460). Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody (ASM10460) at 1:1000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6VO
Other Accession NP_055325.2
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human Piccolo Polyclonal
Target/Specificity Detects ~550kDa. No cross-reactivity to Bassoon.
Other Names ACZ Antibody, Aczonin Antibody, Brain-derived HLMN protein Antibody, DKFZp779G1236 Antibody, KIAA0559 Antibody, Multidomain presynaptic cytomatrix protein Antibody, PCLO Antibody, PCLO_HUMAN Antibody, Piccolo (presynaptic cytomatrix protein) Antibody, Piccolo mouse homolog of Pico Antibody, Presynaptic cytomatrix protein Antibody, Protein piccolo Antibody
Immunogen Full length protein, BC001304.1
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cell Junction | Synapse
Citations (0)

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Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).


1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619.
2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162.
3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

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Cat# ASM10460
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