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TLR4 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, FC, ICC |
|---|---|
| Primary Accession | O00206 |
| Other Accession | NP_612564.1 |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Human TLR4 Polyclonal |
| Target/Specificity | Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag). |
| Other Names | ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody |
| Immunogen | Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4 |
| Purification | Protein A Purified |
| Storage | -20ºC |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization | Membrane |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
References
1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101.
2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298.
3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518(1-2): 157-161.
4. Takeuchi O., et al. (1999) Immunity. 11: 443.
5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167.
6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388(6640): 394-397.
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