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TLR4 Antibody

     
  • IHC - TLR4 Antibody ASM10462
    Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells.
  • WB - TLR4 Antibody ASM10462
    Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:1000.
  • IHC - TLR4 Antibody ASM10462
    Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:100.
  • IHC - TLR4 Antibody ASM10462
    Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (ASM10462) at 1:100.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FACS, FCM, ICC/IF
Primary Accession O00206
Other Accession NP_612564.1
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Description Rabbit Anti-Human TLR4 Polyclonal
Target/Specificity Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Other Names ARMD10 Antibody, CD284 Antibody, TLR 4 Antibody, TOLL Antibody, Toll like receptor 4 Antibody
Immunogen Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 2 µg/ml of SPC-200 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Membrane
Research Areas
Citations (0)

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Background

The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).

References

1. Muzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101.
2. Medzhitov R. and Janeway C.A (1997) Cell. 91: 295-298.
3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta. 1518(1-2): 157-161.
4. Takeuchi O., et al. (1999) Immunity. 11: 443.
5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA. 97: 2163-2167.
6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature. 388(6640): 394-397.

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Cat# ASM10462
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