Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Cytochrome P450 Reductase Antibody   

Cytochrome P450 Reductase Antibody

     
  • ICC/IF - Cytochrome P450 Reductase Antibody ASM10470
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (ASM10470). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (ASM10470) at 1:60 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • ICC/IF - Cytochrome P450 Reductase Antibody ASM10470
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (ASM10470). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (ASM10470) at 1:150 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytosol. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Cytochrome P450 Reductase Antibody. (C) Composite.
  • WB - Cytochrome P450 Reductase Antibody ASM10470
    Western blot analysis of Human, Mouse, Rat Human, Mouse and Rat Lysates showing detection of ~ 75 kDa Cytochrome P450 Reductase protein using Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (ASM10470). Lane 1: MW ladder. Lane 2: Human lysate,  A431. Lane 3: Mouse lung lysate.  Lane 4: Rat lysate, Rat Brain Membrane (RBM). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Cytochrome P450 Reductase Polyclonal Antibody (ASM10470) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP antibody at 1:2000 for 1 hour at RT. Color Development: TMB solution for 11 min at RT. Predicted/Observed Size: ~ 75 kDa. Other Band(s): ~30 -250 in Rat lysate only.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P00388
Other Accession NP_113764.1
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Rat Cytochrome P450 Reductase Polyclonal
Target/Specificity Detects ~77kDa.
Other Names Cytochrome C Reductase Antibody, CCR Antibody, CPR Antibody, P450R Antibody, CYPOR Antibody, DKFZp686G04235 Antibody, FLJ26468 Antibody, NADPH Cytochrome Antibody, P450 Reductase Antibody, NADPH dependent cytochrome P450 reductase Antibody, NADPH-cytochrome P450 reductase Antibody, P450 (cytochrome) oxidoreductase Antibody, P450 Cytochrome Oxidoreductase Antibody, P450R Antibody, POR Antibody
Immunogen Rat native full-length Cytochrome P450 Reductase purified from liver tissue
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-210 was sufficient for detection of Cytochrome P450 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Membrane | Microsome
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Background

Cytochrome P450 Reductase is an enyme that is required for the transfer of electrons from NADPH to cytochrome P450 in microsomes. It has also been found to transfer electrons to Heme oxygenase and cytochrome B5. Cytochrome P450 Reductase is found localized to the endoplasmic reticulum membrane, tethered by its hydrophobic N-terminal tail. Mutations in the gene encoding Cytochrome P450 Reductase (POR) have been found to cause congenital adrenal hyperplasia, Antley-Bixler syndrome, amenorrhea and disordered steroidogenesis.

References

1. Pandey A.V. and Flück C.E. (2013). Pharmacology & therapeutics 138 (2): 229–54.
2. Jensen K. and Møller BL (2010). Phytochemistry 71 (2-3): 132–41.
3. Flück C.E. et al. (2004). Nature Genetics 36 (3): 228–230.

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

Cat# ASM10470
Availability: Inquire
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900
Cedarlane Labs
+1 (800) 721-1644
+1 (336) 513-5138

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"