|Application ||WB, IHC, IEM, ICC/IF|
|Primary Accession||P55018, P55017|
|Other Accession||NP_062218, NP_000330|
|Reactivity||Human, Mouse, Rat, Dog|
|Description||Rabbit Anti-Rat NCC Polyclonal|
|Other Names||SLC12A3 Antibody, SCYL1 Antibody, CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody|
|Immunogen||Poduced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal|
|Purification||Protein A Purified|
|Storage Buffer||PBS, 50% glycerol, 0.09% sodium azide|
|Shipping Temperature||Blue Ice or 4ºC|
|Certificate of Analysis||1 µg/ml of SPC-402 was sufficient for detection of NCC3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|
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Provided below are standard protocols that you may find useful for product applications.
NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712.
2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562.
3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
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