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ENaC gamma Antibody

  • IHC - ENaC gamma Antibody ASM10481
    Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody (ASM10481). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (ASM10481) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).
  • WB - ENaC gamma Antibody ASM10481
    Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (ASM10481). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (ASM10481) at 1:1000.
  • WB - ENaC gamma Antibody ASM10481
    Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (ASM10481). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (ASM10481) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P37091
Other Accession NP_058742
Host Rabbit
Reactivity Human, Mouse, Rat, Hamster, Xenopus
Clonality Polyclonal
Description Rabbit Anti-Rat ENaC gamma Polyclonal
Target/Specificity Detects ~83kDa.
Other Names SCNN1G Antibody, Epithelial Sodium Channel gamma Antibody, Amiloride sensitive epithelial sodium channel gamma subunit Antibody, Amiloride sensitive sodium channel subunit gamma Antibody, Amiloride-sensitive sodium channel subunit gamma Antibody, BESC3 Antibody, ENaC gamma subunit Antibody, ENaCG Antibody, ENaCgamma Antibody, Epithelial Na(+) channel subunit gamma Antibody, Epithelial Na+ channel subunit gamma Antibody, Gamma ENaC Antibody, Gamma NaCH Antibody, Gamma-ENaC Antibody, Gamma-NaCH Antibody, Nonvoltage gated sodium channel 1 subunit gamma Antibody, Nonvoltage-gated sodium channel 1 subunit gamma Antibody, PHA 1 Antibody, PHA1 Antibody, SCNEG Antibody, SCNN 1G Antibody, SCNN1G Antibody, SCNNG_HUMAN Antibody, Sodium channel non voltage gated 1 gamma subunit Antibody, Sodium channel nonvoltage gated 1 gamma Antibody
Immunogen Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-405 was sufficient for detection of gamma-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Apical Cell Membrane
Citations (0)

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The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).


1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689.
2. Gu Y. (2008) J Cell Physiol. 216(2):453-457.
3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609.
4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594.
5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45.
6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.

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Cat# ASM10481
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