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UT-A1 Antibody

     
  • WB - UT-A1 Antibody ASM10482
    Western blot analysis of Rat Inner medulla showing detection of UT-A1 protein using Rabbit Anti-UT-A1 Polyclonal Antibody (ASM10482). Primary Antibody: Rabbit Anti-UT-A1 Polyclonal Antibody (ASM10482) at 1:1000.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q62668
Other Accession NP_062220
Host Rabbit
Reactivity Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Rat UT-A1 Polyclonal
Target/Specificity Detects ~97 and 127kDa.
Other Names SLC14A2 Antibody, FLJ16167 Antibody, hUT-A6 Antibody, HUT2 Antibody, kidney Antibody, MGC119566 Antibody, MGC119567 Antibody, Slc14a2 Antibody, Solute carrier family 14 (urea transporter) Antibody, member 2 Antibody, Solute carrier family 14 member 2 Antibody, Urea transporter 2 Antibody, Urea transporter Antibody, Urea transporter kidney Antibody, UT-A2 Antibody, UT2 Antibody, UT2_HUMAN Antibody, UTA Antibody, UTR Antibody, UT1 Antibody, UTA1 Antibody
Immunogen Produced against a synthetic peptide mapped to the C-terminal tail (amino acids 911-929) of rat UT-A1 (antibody designation L194)
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-406 was sufficient for detection of UT-A1 in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Apical Cell Membrane
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Background

UT-A1, a kidney-specific urea transporter is expressed in the renal collecting duct where it mediates trans-epithelial urea transport and is a target for regulation by vasopressin. Urea movement out of the collecting duct in the inner medulla of the kidney allows accumulation of urea in the medullary interstitium, thereby allowing maximum water reabsorption from the collecting ducts. (The antibody also recognizes a second protein from the UT-A gene driven by transcription from an alternative promoter and expressed in the thin descending limb of Henle, viz. UT-A2) (1, 2).

References

1. Nielsen S., et al. (1996) Proc Natl Acad Sci U S A. 93(11): 5495-500.
2. Smith C.P. (2009) Exp Physiol. 94(2): 180-185.

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Cat# ASM10482
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