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Aquaporin 3 Antibody

     
  • IHC - Aquaporin 3 Antibody ASM10485
    Immunohistochemistry analysis using Rabbit Anti-Aquaporin 3 Polyclonal Antibody (ASM10485). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody (ASM10485) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).
  • WB - Aquaporin 3 Antibody ASM10485
    Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 3 protein using Rabbit Anti-Aquaporin 3 Polyclonal Antibody (ASM10485). Primary Antibody: Rabbit Anti-Aquaporin 3 Polyclonal Antibody (ASM10485) at 1:2000.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P47862
Other Accession NP_113891.1
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Rat Aquaporin 3 Polyclonal
Target/Specificity Detects ~31.5kDa. May detect larger glycosylated bands ~35-50kDa.
Other Names AQP3 Antibody, AQP 3 Antibody, AQP-3 Antibody, Aqp3 Antibody, AQP3_HUMAN Antibody, Aquaglyceroporin-3 Antibody, Aquaporin 3 (GIL blood group) Antibody, Aquaporin 3 (Gill blood group) Antibody, Aquaporin-3 Antibody, Aquaporin3 Antibody, GIL Antibody, Gill blood group Antibody
Immunogen Produced against the C-terminal peptide (Sequence N-CHLEQPPPSTEAENVKLAHMKHKEQI) of rat aquaporin 3
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SPC-504 was sufficient for detection of aquaporin 3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Membrane
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Background

Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin 3 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4).

References

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
3. Sasaki S., Ishibashi K., and Marumo F. (1998) Annu Rev Physiol. 60: 199-220.
4. Dibas A.I., Mia A.J., Yorio T. (1998) Proc Soc Exp Med. 219(3): 183-199.

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Cat# ASM10485
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