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UVRAG Antibody

     
  • ICC/IF - UVRAG Antibody ASM10494
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-UVRAG Polyclonal Antibody (ASM10494). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (ASM10494) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite.
  • WB - UVRAG Antibody ASM10494
    Western blot analysis of Human 293T showing detection of ~70kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (ASM10494). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (ASM10494) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~70kDa.
  • WB - UVRAG Antibody ASM10494
    Western blot analysis of Rat Liver showing detection of ~70kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody (ASM10494). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody (ASM10494) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~70kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9P2Y5
Other Accession NP_003360.2
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human UVRAG Polyclonal
Target/Specificity Predicted molecular weight at ~78.1kDa. Observed molecular weights in the 75-90kDa range.
Other Names DHTX Antibody, p63 Antibody, UV radiation resistance associated Antibody, UVRAG_HUMAN Antibody
Immunogen Synthetic peptide from the C-terminal of human UVRAG
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-605 was sufficient for detection of UVRAG on 293T Rapamycin treated lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Endosome | Lysosome | Endoplasmic reticulum | Nucleus | Chromosome | Centromere
Research Areas
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Background

UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (1). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (2). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (3-4). It is highly expressed in the brain, lung, kidney and liver.

References

1. Liang, C. et al. (2008) Nat Cell Biol. 10: 776-87.
2. Ionov, Y. et al. (2004) Oncogene. 23: 639-45.
3. Kim, M.S. et al. (2008) Hum Pathol. 39: 1059-63.

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Cat# ASM10494
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