|Application ||WB, ICC/IF|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit Anti-Human LC3A Polyclonal|
|Target/Specificity||Detects ~14 kDa. Band at 50 kDa is LC3A complex.|
|Other Names||MAP1 light chain 3 like protein 1 Antibody, Map1lc3a Antibody, MAP1BLC3 Antibody, Autophagy-related ubiquitin-like modifier LC3 A Antibody, MAP1LC3A Antibody, LC3A Antibody, Microtubule-associated proteins 1A and 1B, light chain 3 Antibody, MAP1ALC3 Antibody, MAP1A/1B light chain 3 A Antibody, MAP1A/MAP1B light chain 3 A Antibody, Microtubule-associated protein 1 light chain 3 alpha Antibody, LC3 Antibody, Microtubule-associated proteins 1A/1B light chain 3A Antibody, Autophagy-related protein LC3 A Antibody, MAP1A/MAP1B LC3 A Antibody, ATG8E Antibody, MLP3A_HUMAN Antibody, MAP1 light chain 3-like protein 1 Antibody, Microtubule associated proteins 1A/1B light chain 3 Antibody,|
|Immunogen||Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12)|
|Purification||Peptide Affinity Purified|
|Storage Buffer||PBS, 50% glycerol, 0.09% sodium azide|
|Shipping Temperature||Blue Ice or 4ºC|
|Certificate of Analysis||A 1:1000 dilution of SPC-613 was sufficient for detection of LC3A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|
|Cellular Localization||Cytoplasm | Cytoskeleton | Endomembrane System | Lipid-Anchor | Cytoplasmic Vesicle | Autophagosome Membrane | Lipid-Anchor | Cytoplasmic Vesicle | Autophagosome|
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Provided below are standard protocols that you may find useful for product applications.
Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88.
2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.
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