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GABARAP Antibody

     
  • ICC/IF - GABARAP Antibody  ASM10503
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAP Polyclonal Antibody (ASM10503). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (ASM10503) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Endomembrane System, Cytoplasm, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite.
  • WB - GABARAP Antibody  ASM10503
    Western blot analysis of Human 293T showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (ASM10503). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (ASM10503) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex).
  • WB - GABARAP Antibody  ASM10503
    Western blot analysis of Mouse Kidney showing detection of ~13.9kDa GABARAP protein using Rabbit Anti-GABARAP Polyclonal Antibody (ASM10503). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAP Polyclonal Antibody (ASM10503) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (Complex).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession O95166
Other Accession NP_009209.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human GABARAP Polyclonal
Target/Specificity Detects ~14-16kDa.
Other Names ATG8A Antibody, GABARAP A Antibody, GABA(A) Receptor Associated Protein Antibody, MM46 Antibody
Immunogen Synthetic peptide from the N-terminal of human GABARAP
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-620 was sufficient for detection of GABARAP on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Endomembrane System | Cytoplasm | Cytoskeleton | Golgi Apparatus | Golgi Apparatus Membrane | Cytoplasmic Vesicle
Research Areas
Citations (0)

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Background

Gamma-aminobutyric acid receptor-associated protein, or GABARAP, are lighand gated chloride channels that mediate inhibitory neurotransmission. It clusters neurotransmitter receptors by mediating its interaction with the cytoskeleton (1).

References

1. "Entrez Gene: GABARAP GABA(A) receptor-associated protein".

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Cat# ASM10503
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