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ATG4C Antibody

     
  • ICC/IF - ATG4C Antibody  ASM10513
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10513). Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10513) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
  • WB - ATG4C Antibody  ASM10513
    Western blot analysis of Human 293T showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10513). Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10513) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
  • WB - ATG4C Antibody  ASM10513
    Western blot analysis of Rat Liver showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody (ASM10513). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody (ASM10513) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q96DT6
Other Accession NP_116241.2
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Description Rabbit Anti-Human ATG4C Polyclonal
Target/Specificity Predicted molecular weight at ~52.5kDa. Observed molecular weights at ~45-60kDa.
Other Names APG4 autophagy 4 homolog C Antibody, APG4 C Antibody, Atg4c Antibody, AUTL1 Antibody, Autophagin 3 Antibody, Cysteine protease ATG4C Antibody
Immunogen Synthetic peptide from the N-terminal of human ATG4C
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-632 was sufficient for detection of ATG4C on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm
Research Areas
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Background

ATG4C belongs to the peptidase C54 family. It is a cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. It also has an activity of delipidating enzyme for the PE-conjugated forms (1).

References

1. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.

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Cat# ASM10513
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