|Application ||WB, ICC/IF|
|Reactivity||Human, Mouse, Rat|
|Description||Rabbit Anti-Human SQSTM1 Polyclonal|
|Target/Specificity||Detects ~60 kDa. Band at 40 kDa is isoform 2.|
|Other Names||EBI3-associated protein of 60 kDa Antibody, ORCA Antibody, Osi Antibody, SQSTM 1 Antibody, ZIP 3 Antibody, A170 Antibody, SQSTM1 Antibody, Ubiquitin binding protein p62 Antibody, Protein kinase C-zeta-interacting protein Antibody, FTDALS3 Antibody, Ubiquitin-binding protein p62 Antibody, PKC-zeta-interacting protein Antibody, EBIAP Antibody, Sqstm1 Antibody, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa Antibody, EBI3 associated protein p60 Antibody, EBI3 associated protein of 60 kDa Antibody, SQSTM_HUMAN Antibody, OSF-6 Antibody, Paget disease of bone 3 Antibody, p62B Antibody, p60 Antibody, PDB 3 Antibody, EBI 3 associated protein p60 Antibody, MGC127197 Antibody, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Antibody, EBI 3 associated protein of 60 kDa Antibody, ZIP3 Antibody, STAP Antibody, p62 Antibody, Sequestosome 1 Antibody, OSIL Antibody, STONE14 Antibody, Phosphotyrosine independent ligand for the Lck SH2 domain p62 Antibody, PDB3 Antibody, Oxidative stress induced like Antibody, Sequestosome-1 Antibody, ZIP Antibody,|
|Immunogen||Synthetic peptide from the N-terminal to mid of Human SQSTM1 (aa. 195-205)|
|Purification||Peptide Affinity Purified|
|Storage Buffer||PBS, 50% glycerol, 0.09% sodium azide|
|Shipping Temperature||Blue Ice or 4ºC|
|Certificate of Analysis||A 1:1000 dilution of SPC-636 was sufficient for detection of SQSTM1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|
|Cellular Localization||Cytoplasm | Late Endosome | Lysosome | Cytoplasmic Vesicle | Autophagosome | Nucleus | Endoplasmic Reticulum | Cytoplasm | P-Body|
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Provided below are standard protocols that you may find useful for product applications.
SQSTM1 or Sequestome 1 (p62) is a ubiquitin binding protein that interacts with ubiqutin, providing a scaffold for several signaling proteins and triggering degradation of proteins throught the proteasome or lysossome. This gene regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Mutations in this gene result in sporadic and familial Paget disease of bone. SQSTM1 also binds autophagosomal membrane protein LC3/ATG8.
1. Kirkin V., et al. (2009) Mol Cell. 34: 259-269.
2. Seibenhener M.L., et al. (2007) FEBS Lett. 581: 175-179.
3. Komatsu M., et al. (2010) Nat Cell Biol. 12: 213-223.
4. Bjørkøy G., et al. (2006) Autophagy. 2: 138-139.
5. Pankiv S., et al. (2007) J Biol Chem. 282: 24131-24145.
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