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ULK2 Antibody

     
  • ICC/IF - ULK2 Antibody ASM10521
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle Membrane, Peripheral Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK2 Antibody (D) Composite.
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  • WB - ULK2 Antibody ASM10521
    Western blot analysis of Human HeLa cell lysates showing detection of 112.7 kDa ULK2 protein using Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.7 kDa. Other Band(s): ~70 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q8IYT8
Other Accession NP_001136082.1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Description Rabbit Anti-Human ULK2 Polyclonal
Target/Specificity Detects ~100 kDa.
Other Names Serine/threonine-protein kinase ULK2 Antibody, EC:2.7.11.1 Antibody, Unc 51 (C. elegans) like kinase 2 Antibody, ULK2_HUMAN Antibody, KIAA0623 Antibody, ATG1B Antibody, Serine/threonine protein kinase ULK2 Antibody, Unc-51-like kinase 2 Antibody, Unc 51 like autophagy activating kinase 2 Antibody, Unc51.2 Antibody, ULK2 Antibody, Unc 51 like kinase 2 Antibody,
Immunogen Synthetic peptide from the mid-protein of Human ULK2 (aa. 558-569)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-641 was sufficient for detection of ULK2 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasmic Vesicle Membrane | Peripheral Membrane Protein
Research Areas
Citations (0)
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Background

UNC-51 like kinase 2 (ULK2) is widely expresed and contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. It has been linked to axon growth and is essential for autophagy. Structurally, ULK2 is similar to ATG1.

References

1. Okazaki N., et al. (2000) Brain Res Mol Brain Res. 85: 1-12.
2. Young A.R., et al. (2006) J Cell Sci. 119: 3888-900.
3. Kamada Y., et al. (2000) J Cell Biol. 150: 1507-13.
4. Lee S.B, et al. (2007) EMBO Rep. 8: 360-5.
5. Hara T., et al. (2008) J Cell Biol. 181: 497-510.

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Cat# ASM10521
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