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ULK3 Antibody

     
  • ICC/IF - ULK3 Antibody ASM10522
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK3 Polyclonal Antibody (ASM10522). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (ASM10522) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK3 Antibody (D) Composite.
  • WB - ULK3 Antibody ASM10522
    Western blot analysis of Human A549 cell lysates showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (ASM10522). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (ASM10522) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa. Other Band(s): ~25 kDa in all lysates.
  • WB - ULK3 Antibody ASM10522
    Western blot analysis of Rat Brain cell lysates showing detection of 53.4 kDa ULK3 protein using Rabbit Anti-ULK3 Polyclonal Antibody (ASM10522). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK3 Polyclonal Antibody (ASM10522) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 53.4 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q6PHR2
Other Accession NP_001092906.3
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human ULK3 Polyclonal
Target/Specificity Detects ~53 kDa.
Other Names Serine/threonine-protein kinase ULK3 Antibody, EC:2.7.11.1 Antibody, Ulk3 Antibody, DKFZP434C131 Antibody, Unc-51-like kinase 3 Antibody, FLJ90566 Antibody, ULK3_HUMAN Antibody, unc 51 like kinase 3 (C. elegans) Antibody, ULK3 Antibody,
Immunogen Synthetic peptide from the N-terminal of Human ULK3 (aa. 166-177)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-642 was sufficient for detection of ULK3 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm
Research Areas
Citations (0)

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Background

UNC-51 like kinase 3, ULK3, is a serine-threonine protein kinase that acts as a regulator of SHH signaling and autophagy. Is phosphorylates in vitro GLI2.

References

1. Malovergan A., et al. (2010) J Biol Chem. 285(39): 30079-30090. 2. Young A.R., et al. (2009) Genes Dev. 23(7):798-803.

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Cat# ASM10522
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