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WDR45 Antibody

     
  • ICC/IF - WDR45 Antibody ASM10526
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WDR45 Polyclonal Antibody (ASM10526). Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (ASM10526) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WDR45 Antibody (D) Composite.
  • WB - WDR45 Antibody ASM10526
    Western blot analysis of Human 293Trap cell lysates showing detection of 40 kDa WDR45 protein using Rabbit Anti-WDR45 Polyclonal Antibody (ASM10526). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-WDR45 Polyclonal Antibody (ASM10526) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 40 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9Y484
Other Accession NP_001025067.1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Description Rabbit Anti-Human WDR45 Polyclonal
Target/Specificity Detects ~40 kDa.
Other Names WIPI4 Antibody, WDR45 Antibody, WDRX1 Antibody, WD repeat domain phosphoinositide-interacting protein 4 Antibody, WIPI-4 Antibody, WDRXI4 Antibody, WD repeat-containing protein 45 Antibody, ORF Names: Antibody, JM5 Antibody,
Immunogen Synthetic peptide from the C-terminal of Human WIPI4 (aa. 312-322)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-651 was sufficient for detection of WIPI4 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Research Areas
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Background

WIPI-4 is a 360 amino acid protein containing three WD repeats. Existing as three alternatively spliced isoforms, WIPI-4 is ubiquitously expressed but found at highest levels in heart and skeletal muscle. WD repeat proteins are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Diseases associated with this gene include neurodegeneration with brain iron accululation 5 and 4.

References

1. http://www.genecards.org/cgi-bin/carddisp.pl?gene=WDR45 2. Strausberg R.L., et al. (2002) Proc Natl Acad Sci U S A. 99(26): 16899–16903.
3. Haack T.B., et al. (2012) Am J Hum Genet. 91(6): 1144–1149.

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Cat# ASM10526
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