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ATG13 Antibody

     
  • ICC/IF - ATG13 Antibody  ASM10530
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG13 Polyclonal Antibody (ASM10530). Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody (ASM10530) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm, Cytosol. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG13 Antibody (D) Composite.
  • WB - ATG13 Antibody  ASM10530
    Western blot analysis of Human HEK293 showing detection of ~56.6kDa ATG13 protein using Rabbit Anti-ATG13 Polyclonal Antibody (ASM10530). Lane 1: MW Ladder. Lane 2: Human HEK293 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG13 Polyclonal Antibody (ASM10530) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~56.6kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession O75143
Other Accession NP_001136145.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ATG13 Polyclonal
Target/Specificity Detects ~56-63kDa.
Other Names ATG13_Human Antibody, Autophagy related protein 13 Antibody, KIAA0652 Antibody
Immunogen Synthetic peptide from the mid-protein range of human ATG13
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-657 was sufficient for detection of ATG13 on HEK293 lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cytosol | Preautophagosomal structure
Research Areas
Citations (0)

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Background

ATG13 is an autophagy factor required for phagosome formation. It is a target of the TOR kinase signaling pathway that regulates autophagy through phosphorylation of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (1-3).

References

1. Mercer C.A., Kaliappan A., Dennis P.B. (2009) Autophagy. 5(5): 649-662.
2. Hosokawa N., et al. (2009) Mol Biol Cell. 20(7): 1981-1991.
3. Chan E.Y., Longatti A., McKnight N.C., Tooze S.A. (2009) Mol Cell Biol. 29(1): 157-171.

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Cat# ASM10530
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