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FIP200 Antibody

     
  • ICC/IF - FIP200 Antibody ASM10531
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Nucleus, Cytoplasm, Cytoplasm, Cytosol, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) FIP200 Antibody (D) Composite.
  • WB - FIP200 Antibody ASM10531
    Western blot analysis of Rat Brain cell lysates showing detection of 183 kDa FIP200 protein using Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-FIP200 Polyclonal Antibody (ASM10531) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 183 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q8TDY2
Other Accession NP_001077086.1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Description Rabbit Anti-Human FIP200 Polyclonal
Target/Specificity Detects ~150 kDa.
Other Names RB1CC1 Antibody, FAK family kinase-interacting protein of 200 kDa Antibody, FIP200 Antibody, KIAA0203 Antibody, RBICC Antibody, RB1-inducible coiled-coil protein 1 Antibody
Immunogen Synthetic peptide from the C-terminal of Human FIP200 (aa. 1355-1366)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-658 was sufficient for detection of FIP200 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus | Cytoplasm | Cytoplasm | Cytosol | Preautophagosomal Structure
Research Areas
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Cat# ASM10531
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