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PIK3R4 Antibody

     
  • ICC/IF - PIK3R4 Antibody ASM10532
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-PIK3R4 Polyclonal Antibody (ASM10532). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (ASM10532) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endosome, Cytoplasmic Vesicle, Autophagosome, Membrane, Lipid-Anchor. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PIK3R4 Antibody (D) Composite.
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  • WB - PIK3R4 Antibody ASM10532
    Western blot analysis of Rat Brain cell lysates showing detection of 153.1 kDa PIK3R4 protein using Rabbit Anti-PIK3R4 Polyclonal Antibody (ASM10532). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3R4 Polyclonal Antibody (ASM10532) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 153.1 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q99570
Other Accession NP_055417.1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Description Rabbit Anti-Human PIK3R4 Polyclonal
Target/Specificity Detects ~160 kDa.
Other Names VPS15 Antibody, Phosphoinositide 3-kinase regulatory subunit 4 Antibody, PI3-kinase regulatory subunit 4 Antibody, PI3-kinase p150 subunit Antibody, PIK3R4 Antibody, Phosphoinositide 3-kinase adaptor protein Antibody
Immunogen Synthetic peptide from the C-terminal of Human PIK3R4 (aa. 1314-1325)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-660 was sufficient for detection of PIK3R4 in 15 µg of Rat Brain Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Late Endosome | Cytoplasmic Vesicle | Autophagosome | Membrane | Lipid-Anchor
Research Areas
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Cat# ASM10532
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