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RAB1B Antibody

     
  • WB - RAB1B Antibody ASM10544
    Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 22.1 kDa RAB1B protein using Rabbit Anti-RAB1B Polyclonal Antibody (ASM10544). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAB1B Polyclonal Antibody (ASM10544) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 22.1 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q9H0U4
Other Accession NP_112243.1
Host Rabbit
Reactivity Human, Rat
Clonality Polyclonal
Description Rabbit Anti-Human RAB1B Polyclonal
Target/Specificity Detects ~48 kDa.
Other Names Ras-related protein Rab-1B Antibody, RAB1B Antibody
Immunogen Synthetic peptide from the C-terminal of Human RAB1B (aa. 179-189)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-684 was sufficient for detection of RAB1B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Membrane | Lipid-Anchor | Cytoplasmic Side | Preautophagosomal Structure Membrane | Lipid-Anchor | Cytoplasmic Side
Research Areas
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Cat# ASM10544
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