|Application ||WB, ICC/IF|
|Description||Rabbit Anti-Human BNIP3 Polyclonal|
|Target/Specificity||Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.|
|Other Names||NIP3 Antibody, BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody, BNIP3_Human Antibody|
|Immunogen||Synthetic peptide from the C-terminal of human BNIP3|
|Purification||Peptide Affinity Purified|
|Storage Buffer||PBS, 50% glycerol, 0.09% sodium azide|
|Shipping Temperature||Blue Ice or 4ºC|
|Certificate of Analysis||A 1:1000 dilution of SPC-685 was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|
|Cellular Localization||Mitochondrion | Mitochondrion outer membrane|
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Provided below are standard protocols that you may find useful for product applications.
BNIP3 is an apoptosis-inducing protein which can overcome BCL2 suppression. It may play a role in repartioning calcium between the two major intracellular calcium stores in association with BCL2. It has been shown to interact with CD47, BCL2-like, and BCL-2 (1-3).
1. Boyd J.M., et al. (1994) Cell. 79(2): 341-351.
2. Lamy L., et al. (2003) J Biol Chem. 278(26): 23915-23921.
3. Ray R., et al. (2000) J Biol Chem. 275(2): 1439-1448.
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