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BNIP3 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC/IF |
|---|---|
| Primary Accession | Q12983 |
| Other Accession | NP_004043.3 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Human BNIP3 Polyclonal |
| Target/Specificity | Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3. |
| Other Names | NIP3 Antibody, BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody, BNIP3_Human Antibody |
| Immunogen | Synthetic peptide from the C-terminal of human BNIP3 |
| Purification | Peptide Affinity Purified |
| Storage | -20ºC |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SPC-685 was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization | Mitochondrion | Mitochondrion outer membrane |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
BNIP3 is an apoptosis-inducing protein which can overcome BCL2 suppression. It may play a role in repartioning calcium between the two major intracellular calcium stores in association with BCL2. It has been shown to interact with CD47, BCL2-like, and BCL-2 (1-3).
References
1. Boyd J.M., et al. (1994) Cell. 79(2): 341-351.
2. Lamy L., et al. (2003) J Biol Chem. 278(26): 23915-23921.
3. Ray R., et al. (2000) J Biol Chem. 275(2): 1439-1448.
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