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COX-4 Antibody

     
  • ICC/IF - COX-4 Antibody ASM10548
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-COX-4 Polyclonal Antibody (ASM10548). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (ASM10548) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Mitochondrion. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite.
  • ICC/IF - COX-4 Antibody ASM10548
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-COX-4 Polyclonal Antibody (ASM10548). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (ASM10548) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Mitochondrion Inner Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite.
  • WB - COX-4 Antibody ASM10548
    Western blot analysis of Human A549 cell lysates showing detection of 19.5 kDa COX-4 protein using Rabbit Anti-COX-4 Polyclonal Antibody (ASM10548). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (ASM10548) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 19.5 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P13073
Other Accession NP_001305715.1
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Description Rabbit Anti-Human COX-4 Polyclonal
Target/Specificity Detects ~16 kDa.
Other Names COX4I2 Antibody, COX IV Antibody, COX41_HUMAN Antibody, COX4B Antibody, Cytochrome C Oxidase subunit IV Antibody, Cytochrome c oxydase subunit 4 Antibody, COX4I1 Antibody, AL024441 Antibody, COX4 Antibody, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial Antibody, COX IV-1 Antibody, dJ857M17.2 Antibody, Cytochrome c oxidase subunit IV isoform 1 Antibody, COX IV 1 Antibody, COX4L2 Antibody, Cytochrome c oxidase polypeptide IV Antibody, MGC72016 Antibody, COX 4 Antibody, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Antibody, Cytochrome c oxidase subunit 4 isoform 1, COXIV Antibody, Cox4a Antibody, mitochondrial Antibody, Cytochrome c oxidase subunit IV isoform 2 (lung) Antibody, MGC105470 Antibody, Cox4 Antibody
Immunogen Synthetic peptide of Human COX-4
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-688 was sufficient for detection of COX-4 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion Inner Membrane
Research Areas
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Cat# ASM10548
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