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PCNA Antibody

  • WB - PCNA Antibody ASM10550
    Western blot analysis of Human 293 Trap cell lysates showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (ASM10550). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (ASM10550) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12004
Other Accession NP_002583.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human PCNA Polyclonal
Target/Specificity Detects ~37 kDa.
Other Names cb16 Antibody, MGC8367 Antibody, PCNA_HUMAN Antibody, Polymerase delta accessory protein Antibody, DNA polymerase delta auxiliary protein Antibody, Proliferating cell nuclear antigen Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, PCNAR Antibody, Pcna/cyclin Antibody, HGCN8729 Antibody, wu:fb36g03 Antibody, PCNA Antibody, ATLD2 Antibody, fb36g03 Antibody, OTTHUMP00000030190 Antibody, Cyclin Antibody
Immunogen Synthetic peptide of Human PCNA
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-691 was sufficient for detection of PCNA in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus
Research Areas
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Cat# ASM10550
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