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alpha Tubulin Antibody

     
  • ICC/IF - alpha Tubulin Antibody ASM10551
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha Tubulin Polyclonal Antibody (ASM10551). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-alpha Tubulin Polyclonal Antibody (ASM10551) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Cytoskeleton. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) alpha Tubulin Antibody (D) Composite.
  • WB - alpha Tubulin Antibody ASM10551
    Western blot analysis of Human A549 cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (ASM10551). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (ASM10551) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 50.1 kDa.
  • WB - alpha Tubulin Antibody ASM10551
    Western blot analysis of Human HeLa cell lysates showing detection of 50.1 kDa alpha Tubulin Antibody protein using Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (ASM10551). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-alpha Tubulin Antibody Polyclonal Antibody (ASM10551) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 50.1 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P68363
Other Accession NP_006073.2
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human alpha Tubulin Antibody Polyclonal
Target/Specificity Detects ~50 kDa.
Other Names Alpha-tubulin ubiquitous Antibody, Alpha tubulin ubiquitous Antibody, Tubulin alpha 1B chain Antibody, Tubulin alpha ubiquitous Antibody, Tubulin K-alpha-1 Antibody, TBA1B_HUMAN Antibody, K alpha 1 Antibody, Tubulin alpha ubiquitous chain Antibody, Tubulin alpha 1B Antibody, TUBA1B Antibody, Tubulin alpha-1B chain Antibody, Tubulin alpha-ubiquitous chain Antibody, Tubulin K alpha 1 Antibody
Immunogen Synthetic peptide of Human alpha-Tubulin
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-692 was sufficient for detection of alpha-Tubulin in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cytoskeleton
Research Areas
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Cat# ASM10551
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