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IGF-1 Antibody

     
  • ICC/IF - IGF-1 Antibody ASM10555
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IGF-1 Polyclonal Antibody (ASM10555). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (ASM10555) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IGF-1 Antibody. (D) Composite.
  • WB - IGF-1 Antibody ASM10555
    Western blot analysis of Mouse Liver cell lysates showing detection of ~21.8 kDa IGF-1 protein using Rabbit Anti-IGF-1 Polyclonal Antibody (ASM10555). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IGF-1 Polyclonal Antibody (ASM10555) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~21.8 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P05019
Other Accession NP_000609.1
Host Rabbit
Reactivity Human, Mouse
Clonality Polyclonal
Description Rabbit Anti-Human IGF-1 Polyclonal
Target/Specificity Detects ~25 kDa.
Other Names Insulin-like growth factor 1 Antibody, Igf1 Antibody, IGF-I Antibody, Insulin like growth factor 1 (somatomedin C) Antibody, IGF1_HUMAN Antibody, Somatomedin-C Antibody, MGF Antibody, OTTHUMP00000195083 Antibody, Somatomedia C Antibody, Mechano growth factor Antibody, Insulin like growth factor 1 Antibody, Insulin like growth factor IA Antibody, IGF IB Antibody, IBP1 Antibody, IGF1 Antibody, Insulin-like growth factor I Antibody, IGF IA Antibody, Somatomedin C Antibody, IGF1A Antibody, IGFI Antibody, IGFIA Antibody, IGF I Antibody, Insulin like growth factor IB Antibody, IGF 1 Antibody, IGF I Antibody
Immunogen Synthetic peptide from the mid-protein of Human IGF-1
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-699 was sufficient for detection of IGF-1 in 15 µg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Secreted
Research Areas
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Cat# ASM10555
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