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NRF2 Antibody

     
  • ICC/IF - NRF2 Antibody ASM10564
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-NRF2 Polyclonal Antibody (ASM10564). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-NRF2 Polyclonal Antibody (ASM10564) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Cytosol, Nucleus . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) NRF2 Antibody. (D) Composite.
  • WB - NRF2 Antibody ASM10564
    Western blot analysis of Human HeLa cell lysates showing detection of ~67.8 kDa NRF2 protein using Rabbit Anti-NRF2 Polyclonal Antibody (ASM10564). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-NRF2 Polyclonal Antibody (ASM10564) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~67.8 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q16236
Other Accession NP_001138884.1
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human NRF2 Polyclonal
Target/Specificity Detects ~67.8 kDa.
Other Names Nuclear factor erythroid 2-related factor 2 Antibody, Nrf 2 Antibody, Nuclear factor Antibody, NF2L2_HUMAN Antibody, Nfe2l2 Antibody, Nuclear factor erythroid 2 related factor 2 Antibody, nuclear factor erythroid 2 like 2 Antibody, Nuclear factor (erythroid derived 2) like 2 Antibody, NRF2 Antibody, like 2 Antibody, NF-E2-related factor 2 Antibody, NFE2-related factor 2 Antibody, erythroid derived 2, Nuclear factor, NFE2L2 Antibody, HEBP1 Antibody, NFE2 related factor 2 Antibody, NF E2 related factor 2 Antibody, Nuclear factor erythroid derived 2 like 2 Antibody, erythroid derived 2 Antibody
Immunogen Synthetic peptide from the mid-protein of Human NRF2
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-711 was sufficient for detection of NRF2 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cytosol | Nucleus
Research Areas
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Cat# ASM10564
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