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ADH4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ADH4.

     
  • WB - ADH4 Antibody (monoclonal) (M01) AT1054a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - ADH4 Antibody (monoclonal) (M01) AT1054a
    Western Blot analysis of ADH4 expression in transfected 293T cell line by ADH4 monoclonal antibody (M01), clone 3C5.

    Lane 1: ADH4 transfected lysate(40.2 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - ADH4 Antibody (monoclonal) (M01) AT1054a
    Western blot analysis of ADH4 over-expressed 293 cell line, cotransfected with ADH4 Validated Chimera RNAi ( (Cat # AT1054a )
    detail
  • E - ADH4 Antibody (monoclonal) (M01) AT1054a
    Detection limit for recombinant GST tagged ADH4 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08319
Other Accession NM_000670
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3C5
Calculated MW 40222 Da
Additional Information
Gene ID 127
Other Names Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain, ADH4
Target/Specificity ADH4 (NP_000661, 52 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsADH4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

References

Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia. Jugessur A, et al. PLoS One, 2010 Jul 9. PMID 20634891.Association of ADH4 genetic variants with alcohol dependence risk and related phenotypes: results from a larger multicenter association study. Preuss UW, et al. Addict Biol, 2010 Jul 9. PMID 20626721.A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.Identification of a FOXA-dependent enhancer of human alcohol dehydrogenase 4 (ADH4). Pochareddy S, et al. Gene, 2010 Jul 15. PMID 20363298.Cluster headache is associated with the alcohol dehydrogenase 4 (ADH4) gene. Rainero I, et al. Headache, 2010 Jan. PMID 19925625.

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$ 350.00
Cat# AT1054a
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Availability: 7-10 days
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