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AP2S1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant AP2S1.

     
  • WB - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (41.36 KDa) .
    detail
  • WB - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Western Blot analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody (M01), clone 3E4.

    Lane 1: AP2S1 transfected lysate(17 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Western blot analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi ( (Cat # AT1153a )
    detail
  • E - AP2S1 Antibody (monoclonal) (M01) AT1153a
    Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P53680
Other Accession BC006337
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3E5
Calculated MW 17018 Da
Additional Information
Gene ID 1175
Other Names AP-2 complex subunit sigma, Adaptor protein complex AP-2 subunit sigma, Adaptor-related protein complex 2 subunit sigma, Clathrin assembly protein 2 sigma small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated protein AP17, HA2 17 kDa subunit, Plasma membrane adaptor AP-2 17 kDa protein, Sigma2-adaptin, AP2S1, AP17, CLAPS2
Target/Specificity AP2S1 (AAH06337, 1 a.a. ~ 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAP2S1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts.

References

Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.A conserved dileucine motif mediates clathrin and AP-2-dependent endocytosis of the HIV-1 envelope protein. Byland R, et al. Mol Biol Cell, 2007 Feb. PMID 17108326.Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2. Batonick M, et al. Virology, 2005 Nov 25. PMID 16139856.Human immunodeficiency virus Nef induces rapid internalization of the T-cell coreceptor CD8alphabeta. Stove V, et al. J Virol, 2005 Sep. PMID 16103193.PICK1 interacts with ABP/GRIP to regulate AMPA receptor trafficking. Lu W, et al. Neuron, 2005 Aug 4. PMID 16055064.

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$ 350.00
Cat# AT1153a
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Availability: 7-10 days
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