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ARHGEF1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant ARHGEF1.

     
  • WB - ARHGEF1 Antibody (monoclonal) (M02) AT1184a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.41 KDa) .
    detail
  • WB - ARHGEF1 Antibody (monoclonal) (M02) AT1184a
    Western Blot analysis of ARHGEF1 expression in transfected 293T cell line by ARHGEF1 monoclonal antibody (M02), clone 2D2.

    Lane 1: ARHGEF1 transfected lysate (Predicted MW: 102.4 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - ARHGEF1 Antibody (monoclonal) (M02) AT1184a
    Detection limit for recombinant GST tagged ARHGEF1 is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q92888
Other Accession BC034013
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2D2
Calculated MW 102435 Da
Additional Information
Gene ID 9138
Other Names Rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor, p115-RhoGEF, p115RhoGEF, Sub15, ARHGEF1
Target/Specificity ARHGEF1 (AAH34013.2, 830 a.a. ~ 927 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsARHGEF1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

References

Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.Mutation of ARHGAP9 in patients with coronary spastic angina. Takefuji M, et al. J Hum Genet, 2010 Jan. PMID 19911011.P115 RhoGEF and microtubules decide the direction apoptotic cells extrude from an epithelium. Slattum G, et al. J Cell Biol, 2009 Sep 7. PMID 19720875.Reversible translocation of p115-RhoGEF by G(12/13)-coupled receptors. Meyer BH, et al. J Cell Biochem, 2008 Aug 1. PMID 18320579.

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$ 350.00
Cat# AT1184a
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Availability: 7-10 days
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