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CCNH Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CCNH.

     
  • IF - CCNH Antibody (monoclonal) (M01) AT1422a
    Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - CCNH Antibody (monoclonal) (M01) AT1422a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
    detail
  • WB - CCNH Antibody (monoclonal) (M01) AT1422a
    CCNH monoclonal antibody (M01), clone 1B8 Western Blot analysis of CCNH expression in HeLa ( (Cat # AT1422a )
    detail
  • WB - CCNH Antibody (monoclonal) (M01) AT1422a
    Western Blot analysis of CCNH expression in transfected 293T cell line by CCNH monoclonal antibody (M01), clone 1B8.

    Lane 1: CCNH transfected lysate(37.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - CCNH Antibody (monoclonal) (M01) AT1422a
    Immunoperoxidase of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
    detail
  • E - CCNH Antibody (monoclonal) (M01) AT1422a
    Detection limit for recombinant GST tagged CCNH is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession P51946
Other Accession BC005280
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a kappa
Clone Names 1B8
Calculated MW 37643 Da
Additional Information
Gene ID 902
Other Names Cyclin-H, MO15-associated protein, p34, p37, CCNH
Target/Specificity CCNH (AAH05280, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCCNH Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

References

1.Mutations in UVSSA cause UV-sensitive syndrome and impair RNA polymerase IIo processing in transcription-coupled nucleotide-excision repair.Nakazawa Y, Sasaki K, Mitsutake N, Matsuse M, Shimada M, Nardo T, Takahashi Y, Ohyama K, Ito K, Mishima H, Nomura M, Kinoshita A, Ono S, Takenaka K, Masuyama R, Kudo T, Slor H, Utani A, Tateishi S, Yamashita S, Stefanini M, Lehmann AR, Yoshiura KI, Ogi T.Nat Genet. 2012 Apr 1. doi: 10.1038/ng.2229. [Epub ahead of print]

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$ 350.00
Cat# AT1422a
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Availability: 7-10 days
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