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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Ubiquitin E3 Ligases (RING Domain) Antibodies   >   CHFR Antibody (monoclonal) (M01)   

CHFR Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant CHFR.

  • WB - CHFR Antibody (monoclonal) (M01) AT1518a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (97.46 KDa) .
  • IHC - CHFR Antibody (monoclonal) (M01) AT1518a
    Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2 ug/ml]
  • E - CHFR Antibody (monoclonal) (M01) AT1518a
    Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.
  • WB - CHFR Antibody (monoclonal) (M01) AT1518a
    Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( (Cat # AT1518a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96EP1
Other Accession BC012072
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1H3-A12
Calculated MW 73386 Da
Additional Information
Gene ID 55743
Other Names E3 ubiquitin-protein ligase CHFR, 632-, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196, CHFR, RNF196
Target/Specificity CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCHFR Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1.CHFR regulates the mitotic checkpoint by targeting PARP-1 for ubiquitination and degradation.Kashima L, Idogawa M, Mita H, Shitashige M, Yamada T, Ogi K, Suzuki H, Toyota M, Ariga H, Sasaki Y, Tokino T.J Biol Chem. 2012 Feb 15. [Epub ahead of print]2.Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach.Fujita A, Yamamoto H, Imamura M, Nakamura N, Maehara Y, Tsuneyoshi M, Oda Y.Virchows Arch. 2011 Dec 22.3.Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer.Hu SL, Huang DB, Sun YB, Wu L, Xu WP, Yin S, Chen J, Jiang XD, Shen G.Med Oncol. 2011 Jun;28(2):447-54. Epub 2010 Mar 19.4.Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma.Gao YJ, Xin Y, Zhang JJ, Zhou J.World J Gastroenterol. 2008 Aug 28;14(32):5000-7.5.Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint.Privette LM, Weier JF, Nguyen HN, Yu X, Petty EM.Neoplasia. 2008 Jul;10(7):643-52.6.Altered Expression of the Early Mitotic Checkpoint Protein, CHFR, in Breast Cancers: Implications for Tumor Suppression.Privette LM, Gonzalez ME, Ding L, Kleer CG, Petty EM.Cancer Res. 2007 Jul 1;67(13):6064-74. Epub 2007 Jun 27.7.Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis.Milne AN, Sitarz R, Carvalho R, Polak MM, Ligtenberg M, Pauwels P, Offerhaus GJ, Weterman MA.Hum Pathol. 2007 Jun;38(6):903-13. Epub 2007 Mar 21.

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$ 350.00
Cat# AT1518a
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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