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CLIC3 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant CLIC3.

     
  • IF - CLIC3 Antibody (monoclonal) (M02) AT1557a
    Immunofluorescence of monoclonal antibody to CLIC3 on HeLa cell. [antibody concentration 15 ug/ml]
    detail
  • IP - CLIC3 Antibody (monoclonal) (M02) AT1557a
    Immunoprecipitation of CLIC3 transfected lysate using anti-CLIC3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CLIC3 MaxPab rabbit polyclonal antibody.
    detail
  • WB - CLIC3 Antibody (monoclonal) (M02) AT1557a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - CLIC3 Antibody (monoclonal) (M02) AT1557a
    CLIC3 monoclonal antibody (M02), clone 3F8 Western Blot analysis of CLIC3 expression in A-431 ( (Cat # AT1557a )
    detail
  • WB - CLIC3 Antibody (monoclonal) (M02) AT1557a
    Western Blot analysis of CLIC3 expression in transfected 293T cell line by CLIC3 monoclonal antibody (M02), clone 3F8.

    Lane 1: CLIC3 transfected lysate(26.6 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - CLIC3 Antibody (monoclonal) (M02) AT1557a
    Immunoperoxidase of monoclonal antibody to CLIC3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
    detail
  • IHC - CLIC3 Antibody (monoclonal) (M02) AT1557a
    Immunoperoxidase of monoclonal antibody to CLIC3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
    detail
  • E - CLIC3 Antibody (monoclonal) (M02) AT1557a
    Detection limit for recombinant GST tagged CLIC3 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, IP, E
Primary Accession O95833
Other Accession NM_004669
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3F8
Calculated MW 26648 Da
Additional Information
Gene ID 9022
Other Names Chloride intracellular channel protein 3, CLIC3
Target/Specificity CLIC3 (NP_004660, 137 a.a. ~ 236 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCLIC3 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 3 is a member of the p64 family and is predominantly localized in the nucleus and stimulates chloride ion channel activity. In addition, this protein may participate in cellular growth control, based on its association with ERK7, a member of the MAP kinase family.

References

Structure of human CLIC3 at 2 A resolution. Littler DR, et al. Proteins, 2010 May 1. PMID 20146363.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Expression and cellular localisation of chloride intracellular channel 3 in human placenta and fetal membranes. Money TT, et al. Placenta, 2007 May-Jun. PMID 17027078.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.

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$ 350.00
Cat# AT1557a
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Availability: 7-10 days
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