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CNOT3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CNOT3.

     
  • IF - CNOT3 Antibody (monoclonal) (M01) AT1572a
    Immunofluorescence of monoclonal antibody to CNOT3 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - CNOT3 Antibody (monoclonal) (M01) AT1572a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - CNOT3 Antibody (monoclonal) (M01) AT1572a
    CNOT3 monoclonal antibody (M01), clone 4B8 Western Blot analysis of CNOT3 expression in Hela S3 NE ( (Cat # AT1572a )
    detail
  • WB - CNOT3 Antibody (monoclonal) (M01) AT1572a
    Western Blot analysis of CNOT3 expression in transfected 293T cell line by CNOT3 monoclonal antibody (M01), clone 4B8.

    Lane 1: CNOT3 transfected lysate(82 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - CNOT3 Antibody (monoclonal) (M01) AT1572a
    Detection limit for recombinant GST tagged CNOT3 is 0.3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O75175
Other Accession NM_014516
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 4B8
Calculated MW 81872 Da
Additional Information
Gene ID 4849
Other Names CCR4-NOT transcription complex subunit 3, CCR4-associated factor 3, Leukocyte receptor cluster member 2, CNOT3, KIAA0691, LENG2, NOT3
Target/Specificity CNOT3 (NP_055331, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCNOT3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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References

1.Identification of Ccr4-Not complex components as regulators of transition from partial to genuine induced pluripotent stem cells.Kamon M, Katano M, Hiraki-Kamon K, Hishida T, Nakachi Y, Mizuno Y, Okazaki Y, Suzuki A, Hirasaki M, Ueda A, Nishimoto M, Kato H, Okuda AStem Cells Dev. 2013 Nov 8.2.The Anti-Proliferative Activity of BTG/TOB Proteins Is Mediated via the Caf1a (CNOT7) and Caf1b (CNOT8) Deadenylase Subunits of the Ccr4-Not Complex.Doidge R, Mittal S, Aslam A, Winkler GS.PLoS One. 2012;7(12):e51331. doi: 10.1371/journal.pone.0051331. Epub 2012 Dec 7.3.The Ccr4-not deadenylase subunits CNOT7 and CNOT8 have overlapping roles and modulate cell proliferation.Aslam A, Mittal S, Koch F, Andrau JC, Winkler GS.Mol Biol Cell. 2009 Sep;20(17):3840-50. Epub 2009 Jul 15.

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$ 350.00
Cat# AT1572a
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Availability: 7-10 days
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