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DDX20 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant DDX20.

     
  • IF - DDX20 Antibody (monoclonal) (M01) AT1733a
    Immunofluorescence of monoclonal antibody to DDX20 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • E - DDX20 Antibody (monoclonal) (M01) AT1733a
    Detection limit for recombinant GST tagged DDX20 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, E
Primary Accession Q9UHI6
Other Accession NM_007204
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5H5
Calculated MW 92241 Da
Additional Information
Gene ID 11218
Other Names Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein 20, DEAD box protein DP 103, Gemin-3, DDX20, DP103, GEMIN3
Target/Specificity DDX20 (NP_009135, 725 a.a. ~ 824 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDDX20 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.

References

SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro. Todd AG, et al. J Mol Biol, 2010 Sep 3. PMID 20620147.Role of primary miRNA polymorphic variants in metastatic colon cancer patients treated with 5-fluorouracil and irinotecan. Boni V, et al. Pharmacogenomics J, 2010 Jun 29. PMID 20585341.Black carbon exposures, blood pressure, and interactions with single nucleotide polymorphisms in MicroRNA processing genes. Wilker EH, et al. Environ Health Perspect, 2010 Jul. PMID 20211803.Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair. Svendsen JM, et al. Cell, 2009 Jul 10. PMID 19596235.Genetic variations in microRNA-related genes are novel susceptibility loci for esophageal cancer risk. Ye Y, et al. Cancer Prev Res (Phila), 2008 Nov. PMID 19138993.

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$ 350.00
Cat# AT1733a
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Availability: 7-10 days
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