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DDX43 Antibody (monoclonal) (M07)

Mouse monoclonal antibody raised against a partial recombinant DDX43.

     
  • IF - DDX43 Antibody (monoclonal) (M07) AT1737a
    Immunofluorescence of monoclonal antibody to DDX43 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - DDX43 Antibody (monoclonal) (M07) AT1737a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.53 KDa) .
    detail
  • WB - DDX43 Antibody (monoclonal) (M07) AT1737a
    Western Blot analysis of DDX43 expression in transfected 293T cell line by DDX43 monoclonal antibody (M07), clone 3G12.

    Lane 1: DDX43 transfected lysate(72.9 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - DDX43 Antibody (monoclonal) (M07) AT1737a
    Detection limit for recombinant GST tagged DDX43 is 0.3 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9NXZ2
Other Accession NM_018665
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3G12
Calculated MW 72844 Da
Additional Information
Gene ID 55510
Other Names Probable ATP-dependent RNA helicase DDX43, Cancer/testis antigen 13, CT13, DEAD box protein 43, DEAD box protein HAGE, Helical antigen, DDX43, HAGE
Target/Specificity DDX43 (NP_061135.1, 2 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDDX43 Antibody (monoclonal) (M07) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is an ATP-dependent RNA helicase in the DEAD-box family and displays tumor-specific expression.

References

HAGE, a cancer/testis antigen expressed at the protein level in a variety of cancers. Mathieu MG, et al. Cancer Immun, 2010 Jan 11. PMID 20058853.HAGE, a cancer/testis antigen with potential for melanoma immunotherapy: identification of several MHC class I/II HAGE-derived immunogenic peptides. Mathieu MG, et al. Cancer Immunol Immunother, 2007 Dec. PMID 17487488.Epigenetic regulation of human cancer/testis antigen gene, HAGE, in chronic myeloid leukemia. Roman-Gomez J, et al. Haematologica, 2007 Feb. PMID 17296563.The LIFEdb database in 2006. Mehrle A, et al. Nucleic Acids Res, 2006 Jan 1. PMID 16381901.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.

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$ 350.00
Cat# AT1737a
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Availability: 7-10 days
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