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DHFR Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant DHFR.

  • WB - DHFR Antibody (monoclonal) (M01) AT1758a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - DHFR Antibody (monoclonal) (M01) AT1758a
    DHFR monoclonal antibody (M01), clone 2B10 Western Blot analysis of DHFR expression in HeLa ( (Cat # AT1758a )
  • WB - DHFR Antibody (monoclonal) (M01) AT1758a
    DHFR monoclonal antibody (M01), clone 2B10. Western Blot analysis of DHFR expression in PC-12 ( (Cat # AT1758a )
  • WB - DHFR Antibody (monoclonal) (M01) AT1758a
    Western Blot analysis of DHFR expression in transfected 293T cell line by DHFR monoclonal antibody (M01), clone 2B10.

    Lane 1: DHFR transfected lysate(21.5 KDa).
    Lane 2: Non-transfected lysate.
  • IF - DHFR Antibody (monoclonal) (M01) AT1758a
    Immunofluorescence of monoclonal antibody to DHFR on HeLa cell. [antibody concentration 10 ug/ml]
  • E - DHFR Antibody (monoclonal) (M01) AT1758a
    Detection limit for recombinant GST tagged DHFR is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00374
Other Accession BC003584
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2B10
Calculated MW 21453 Da
Additional Information
Gene ID 1719
Other Names Dihydrofolate reductase, DHFR
Target/Specificity DHFR (AAH03584, 88 a.a. ~ 187 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDHFR Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.


1.Influence of Reduced Folate Carrier and Dihydrofolate Reductase Genes on Methotrexate-Induced Cytotoxicity.Yoon SA, Choi JR, Kim JO, Shin JY, Zhang X, Kang JH.Cancer Res Treat. 2010 Sep;42(3):163-71. Epub 2010 Sep 30.2.Telomere capping in non-dividing yeast cells requires Yku and Rap1.Vodenicharov MD, Laterreur N, Wellinger RJ.EMBO J. 2010 Jul 13. [Epub ahead of print]3.Anticancer drug encapsulated in inorganic lattice can overcome drug resistance.Choi SJ, Choi GE, Oh JM, Oh YJ, Park MC, Choy JH.J. Mater. Chem., 2010, 20, 9463-94694.Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes.Ionova IA, Vasquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GM.Am J Physiol Heart Circ Physiol. 2008 Nov;295(5):H2178-87. Epub 2008 Oct 3.

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$ 350.00
Cat# AT1758a
Availability: 7-10 days
Bulk Size
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