DNAI2 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant DNAI2.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, E |
---|---|
Primary Accession | Q9GZS0 |
Other Accession | BC039582 |
Reactivity | Human |
Host | mouse |
Clonality | Monoclonal |
Isotype | IgG2a kappa |
Clone Names | 1C8 |
Calculated MW | 68821 Da |
Gene ID | 64446 |
---|---|
Other Names | Dynein intermediate chain 2, axonemal, Axonemal dynein intermediate chain 2, DNAI2 |
Target/Specificity | DNAI2 (AAH39582, 1 a.a. ~ 593 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Dilution | WB~~1:500~1000 |
Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
Precautions | DNAI2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Background
The protein encoded by this gene belongs to the dynein intermediate chain family, and is part of the dynein complex of respiratory cilia and sperm flagella. Mutations in this gene are associated with primary ciliary dyskinesia type 9. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
References
1.Pih1d3 is required for cytoplasmic preassembly of axonemal dynein in mouse sperm.Dong F, Shinohara K, Botilde Y, Nabeshima R, Asai Y, Fukumoto A, Hasegawa T, Matsuo M, Takeda H, Shiratori H, Nakamura T, Hamada HJ Cell Biol. 2014 Jan 20;204(2):203-13. doi: 10.1083/jcb.201304076. Epub 2014 Jan 13.2.Mutations in DNAH1, which Encodes an Inner Arm Heavy Chain Dynein, Lead to Male Infertility from Multiple Morphological Abnormalities of the Sperm Flagella.Ben Khelifa M, Coutton C, Zouari R, Karaouzene T, Rendu J, Bidart M, Yassine S, Pierre V, Delaroche J, Hennebicq S, Grunwald D, Escalier D, Pernet-Gallay K, Jouk PS, Thierry-Mieg N, Toure A, Arnoult C, Ray PFAm J Hum Genet. 2014 Jan 2;94(1):95-104. doi: 10.1016/j.ajhg.2013.11.017. Epub 2013 Dec 19.3.DYX1C1 is required for axonemal dynein assembly and ciliary motility.Tarkar A, Loges NT, Slagle CE, Francis R, Dougherty GW, Tamayo JV, Shook B, Cantino M, Schwartz D, Jahnke C, Olbrich H, Werner C, Raidt J, Pennekamp P, Abouhamed M, Hjeij R, Kohler G, Griese M, Li Y, Lemke K, Klena N, Liu X, Gabriel G, Tobita K, Jaspers M, Morgan LC, Shapiro AJ, Letteboer SJ, Mans DA, Carson JL, Leigh MW, Wolf WE, Chen S, Lucas JS, Onoufriadis A, Plagnol V, Schmidts M, Boldt K, Roepman R, Zariwala MA, Lo CW, Mitchison HM, Knowles MR, Burdine RD, Loturco JJ, Omran HNat Genet. 2013 Jul 21. doi: 10.1038/ng.2707.4.Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins.Omran H, Kobayashi D, Olbrich H, Tsukahara T, Loges NT, Hagiwara H, Zhang Q, Leblond G, O'Toole E, Hara C, Mizuno H, Kawano H, Fliegauf M, Yagi T, Koshida S, Miyawaki A, Zentgraf H, Seithe H, Reinhardt R, Watanabe Y, Kamiya R, Mitchell DR, Takeda H.Nature. 2008 Dec 4;456(7222):611-6.5.DNAI2 Mutations Cause Primary Ciliary Dyskinesia with Defects in the Outer Dynein Arm.Loges NT, Olbrich H, Fenske L, Mussaffi H, Horvath J, Fliegauf M, Kuhl H, Baktai G, Peterffy E, Chodhari R, Chung EM, Rutman A, O'Callaghan C, Blau H, Tiszlavicz L, Voelkel K, Witt M, Zietkiewicz E, Neesen J, Reinhardt R, Mitchison HM, Omran H.Am J Hum Genet. 2008 Nov;83(5):547-58. Epub 2008 Oct 23.
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