|Application ||WB, IHC, IF, E|
|Calculated MW||26563 Da|
|Other Names||Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTPase, dUTP pyrophosphatase, DUT|
|Target/Specificity||DUT (AAH33645, 68 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||DUT Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
dUTP pyrophosphatase expression correlates with a poor prognosis in hepatocellular carcinoma. Takatori H, et al. Liver Int, 2010 Mar. PMID 19968781.Kinetic properties and specificity of trimeric Plasmodium falciparum and human dUTPases. Quesada-Soriano I, et al. Biochimie, 2010 Feb. PMID 19879316.Polymorphisms in uracil-processing genes, but not one-carbon nutrients, are associated with altered DNA uracil concentrations in an urban Puerto Rican population. Chanson A, et al. Am J Clin Nutr, 2009 Jun. PMID 19403629.Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomers. Tak?cs E, et al. FEBS Lett, 2009 Mar 4. PMID 19302784.An empirical framework for binary interactome mapping. Venkatesan K, et al. Nat Methods, 2009 Jan. PMID 19060904.
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