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EHMT1 Antibody (monoclonal) (M05)

Mouse monoclonal antibody raised against a partial recombinant EHMT1.

     
  • IF - EHMT1 Antibody (monoclonal) (M05) AT1869a
    Immunofluorescence of monoclonal antibody to EHMT1 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - EHMT1 Antibody (monoclonal) (M05) AT1869a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - EHMT1 Antibody (monoclonal) (M05) AT1869a
    EHMT1 monoclonal antibody (M05), clone 3G1 Western Blot analysis of EHMT1 expression in Hela S3 NE ( (Cat # AT1869a )
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession Q9H9B1
Other Accession NM_024757
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3G1
Calculated MW 141466 Da
Additional Information
Gene ID 79813
Other Names Histone-lysine N-methyltransferase EHMT1, 211-, Euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, G9a-like protein 1, GLP, GLP1, Histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, Lysine N-methyltransferase 1D, EHMT1, EUHMTASE1, GLP, KIAA1876, KMT1D
Target/Specificity EHMT1 (NP_079033, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsEHMT1 Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
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Background

The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome). Two transcript variants encoding different isoforms have been found for this gene.

References

MDM2 recruitment of lysine methyltransferases regulates p53 transcriptional output. Chen L, et al. EMBO J, 2010 Aug 4. PMID 20588255.A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.G9a and Glp methylate lysine 373 in the tumor suppressor p53. Huang J, et al. J Biol Chem, 2010 Mar 26. PMID 20118233.Structural biology of human H3K9 methyltransferases. Wu H, et al. PLoS One, 2010 Jan 11. PMID 20084102.

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$ 350.00
Cat# AT1869a
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