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DYKDDDDK Tag Eluting Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Calculated MW | 1012.91 Da |
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| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP1013a is DYKDDDDK (Same epitope as Sigma's Anti-FLAG? M2 Antibody). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
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| Format | The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). Abgent锟渟 DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG锟?antibodies. (FLAG锟?is a registered trademark of Sigma-Aldrich Co., which is not affiliated with Abgent).
References
Chubet RG, Brizzard BL. Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells. Biotechniques 1996;20(1):136-141
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