|Other Names||RAS guanyl-releasing protein 2, Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, Cdc25-like protein, hCDC25L, F25B33 kinase-like protein, RASGRP2, CDC25L, MCG7|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.|
|Cellular Location||Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Cell projection, ruffle membrane; Peripheral membrane protein. Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons (By similarity)|
|Tissue Location||Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a brain-enrichednucleotide exchanged factor that contains an N-terminal GEF domain,2 tandem repeats of EF-hand calcium-binding motifs, and aC-terminal diacylglycerol/phorbol ester-binding domain. Thisprotein can activate small GTPases, including RAS and RAP1/RAS3.The nucleotide exchange activity of this protein can be stimulatedby calcium and diacylglycerol. Three alternatively splicedtranscript variants encoding the same protein have been found forthis gene.
Nagamine, K., et al. Biol. Pharm. Bull. 33(7):1138-1142(2010)Katagiri, K., et al. J. Biol. Chem. 279(12):11875-11881(2004)Eto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 99(20):12819-12824(2002)Toki, S., et al. J. Comp. Neurol. 437(4):398-407(2001)Guo, F.F., et al. J. Biol. Chem. 276(27):25568-25581(2001)
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