TRIM13 Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O60858 |
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Clone Names | 100408307 |
Gene ID | 10206 |
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Other Names | E3 ubiquitin-protein ligase TRIM13, 632-, B-cell chronic lymphocytic leukemia tumor suppressor Leu5, Leukemia-associated protein 5, Putative tumor suppressor RFP2, RING finger protein 77, Ret finger protein 2, Tripartite motif-containing protein 13, TRIM13, LEU5, RFP2, RNF77 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13285a was selected from the N-term region of TRIM13. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | TRIM13 |
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Synonyms | LEU5, RFP2, RNF77 |
Function | Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER- associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress- induced autophagy, and may act as a tumor suppressor (PubMed:22178386). Also plays a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation (PubMed:28087809). Participates as well in T-cell receptor- mediated NF-kappa-B activation (PubMed:25088585). In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B (PubMed:25152375). |
Cellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein Note=Concentrates and colocalizes with p62/SQSTM1 and ZFYVE1 at the perinuclear endoplasmic reticulum |
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the tripartite motif (TRIM)family. The TRIM motif includes three zinc-binding domains, a RING,a B-box type 1 and a B-box type 2, and a coiled-coil region. Thisgene is located on chromosome 13 within the minimal deletion regionfor B-cell chronic lymphocytic leukemia. Multiple alternativelyspliced transcript variants have been found for this gene.
References
Lerner, M., et al. Mol. Biol. Cell 18(5):1670-1682(2007)Skoblov, M., et al. Biochem. Biophys. Res. Commun. 342(3):859-866(2006)Corcoran, M.M., et al. Genes Chromosomes Cancer 40(4):285-297(2004)Dunham, A., et al. Nature 428(6982):522-528(2004)van Everdink, W.J., et al. Cancer Genet. Cytogenet. 146(1):48-57(2003)
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