|Other Names||Dual specificity phosphatase 28, DUSP28|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8925c was selected from the Center region of human DUSP28. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Has phosphatase activity with the synthetic substrate 6,8- difluoro-4-methylumbelliferyl phosphate (in vitro) (PubMed:24531476, PubMed:29121083). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine (PubMed:29121083). The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site (PubMed:29121083).|
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Provided below are standard protocols that you may find useful for product applications.
DUSP28 belongs to the protein-tyrosine phosphatase family, non-receptor class dual specificity subfamily. It contains one tyrosine-protein phosphatase domain.
Strausberg,R.L.,et.al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
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