|Other Names||Chromatin assembly factor 1 subunit B, CAF-1 subunit B, Chromatin assembly factor I p60 subunit, CAF-I 60 kDa subunit, CAF-I p60, M-phase phosphoprotein 7, CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.|
|Cellular Location||Nucleus. Cytoplasm. Note=DNA replication foci. Cytoplasmic in M phase|
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Provided below are standard protocols that you may find useful for product applications.
CHAF1B is required for the assembly of histone octamers onto newly-replicated DNA. CHAF1B is composed of three protein subunits, p50, p60, and p150. The protein corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
Loyola,A., et.al., EMBO Rep. 10 (7), 769-775 (2009)Staibano,S., et.al., Histopathology 54 (5), 580-589 (2009)Morita,M., et.al., Mol. Cell. Biol. 27 (13), 4980-4990 (2007)
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