Caspase-2, human recombinant protein
Caspase-2
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P42575 |
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Calculated MW | two large (19 kDa) and two small (12 kDa) subunits |
Gene ID | 835 |
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Gene Symbol | CASP2 |
Other Names | Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1 |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Caspase-2 |
Application Notes | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
Format | Lyophilized powder |
Storage | -70°C; Lyophilized powder |
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Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-2 (also know as Ich-1, Nedd-2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis pro-caspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed active caspase-2 is a heterotetramer consisting of two large (19 kDa) and two small (12 kDa) subunits.The recombinant active human caspase-2 was expressed in E. coli. The active caspase-2 preferentially cleaves caspase-2 substrates (e.g., VDVAD-AFC or VDVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VDVAD-pNA or Ac-VDVAD-AFC
References
Wang L.,et al.Cell 78:739-750(1994).
Droin N.,et al.Cancer Res. 60:7039-7047(2000).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 424:157-164(2003).
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