Caspase-4, human recombinant protein
Caspase-4
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P49662 |
---|---|
Calculated MW | 43262 Da |
Gene ID | 837 |
---|---|
Gene Symbol | CASP4 |
Other Names | Caspase-4, CASP-4, ICE(rel)-II, Protease ICH-2, Protease TX |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Caspase-4 |
Application Notes | Reconstitute to 1 unit per µl in PBS containing 15% glycerol. |
Format | Lyophilized powder |
Storage | -70°C; Lyophilized powder |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Caspase-4 (also know as Ich-4, ICErelII, TX) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-4 exists in cells as an inactive proenzyme which is matured by proteolysis to yield large and small subunits. The active caspase-4 is a heterotetramer consisting of two large and two small subunits. To date the mechanism of caspase-4 activation and roles of caspase-4 in apoptosis are poorly understood. The recombinant active human caspase-4 was expressed in E. coli. The active caspase-4 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-WEHD-pNA or Ac-WEHD-AFC
References
Faucheu C.,et al.EMBO J. 14:1914-1922(1995).
Munday N.A.,et al.J. Biol. Chem. 270:15870-15876(1995).
Kamens J.,et al.J. Biol. Chem. 270:15250-15256(1995).
Fernandes-Alnemri T.,et al.Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
Taylor T.D.,et al.Nature 440:497-500(2006).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.