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Active Porcine Calpain 1 recombinant protein
Calpain 1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P35750 |
|---|---|
| Calculated MW | 110 kDa |
| Gene ID | 397027 |
|---|---|
| Gene Symbol | CAPN1 |
| Other Names | Calpain-1 catalytic subunit, Calcium-activated neutral proteinase 1, CANP 1, Calpain mu-type, Calpain-1 large subunit, Micromolar-calpain |
| Gene Source | Porcine |
| Source | Porcine Erythrocytes |
| Assay&Purity | SDS-PAGE; ≥85% |
| Assay2&Purity2 | HPLC; |
| Recombinant | No |
| Results | ≥ 1000 units/mg |
| Target/Specificity | Calpain 1 |
| Format | Liquid |
| Storage | -70°C; In 20 mM imidazole-HCl, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The activity of Calpain activity is attributed to two main isoforms: n-calpain and m-calpain, which are ubiquitously, expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (n-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. CAPN1 is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of the substrates involved in cytoskeletal remodeling and signal transduction. CAPN1 is activated by micromolar concentrations of calcium and inhibited by calpastatin.
References
Smith T.P.L.,et al.J. Anim. Sci. 79:552-553(2001).
Winteroe A.K.,et al.Mamm. Genome 7:509-517(1996).
Sun W.,et al.Biochimie 75:931-936(1993).
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