BDNF, human recombinant protein
Brain Derived Neurotrophic Factor
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P23560 |
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Calculated MW | 27.0 kDa |
Gene ID | 627 |
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Gene Symbol | BDNF |
Other Names | Brain-derived neurotrophic factor, Short name=BDNF, Abrineurin |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Results | 0.1-1.0 ng/ml. |
Target/Specificity | BDNF |
Application Notes | Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. |
Format | Lyophilized protein |
Storage | -20°C; Sterile filtered and lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
Human Brain-Derived Neurotrophic Factor (BDGF) is a potent neurotrophic factor that supports the growth and survivability of nerve and glial cells. The active form of BDNF is a 27.0 kDa dimer, formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions.
References
Jones K.R.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:8060-8064(1990).
Maisonpierre P.C.,et al.Genomics 10:558-568(1991).
Shintani A.,et al.Biochem. Biophys. Res. Commun. 182:325-332(1992).
Liu Q.-R.,et al.Am. J. Med. Genet. B Neuropsychiatr. Genet. 134:93-103(2005).
Pruunsild P.,et al.Genomics 90:397-406(2007).
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