Procalcitonin, human recombinant protein
Procalcitonin, calcitonin, CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; MGC126648
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P01258 |
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Calculated MW | 13. 2 kDa |
Gene ID | 796 |
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Other Names | Procalcitonin, calcitonin, CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; MGC126648 |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥97% |
Assay2&Purity2 | HPLC; ≥97% |
Recombinant | Yes |
Target/Specificity | Procalcitonin |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile H₂O to a concentration of 0.1 mg/ml. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from a 10 mM sodium phosphate buffer, pH 7.5. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, an N terminal residue, calcitonin and katacalcin. Levels of unprocessed Procalcitonin rise significantly after bacterial infection, trauma or shock. Recombinant Human Procalcitonin produced in E.coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids with a molecular weight of 12,796 Daltons.
References
le Moullec J.-M.,et al.FEBS Lett. 167:93-97(1984).
Jonas V.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:1994-1998(1985).
Craig R.K.,et al.Biochem. Soc. Symp. 52:91-105(1986).
Riley J.H.,et al.FEBS Lett. 198:71-79(1986).
Minvielle S.,et al.J. Biol. Chem. 266:24627-24631(1991).
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