CD40Ligand/TRAP, human recombinant protein
CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T c
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P29965 |
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Calculated MW | 16.3 kDa |
Gene ID | 959 |
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Gene Symbol | CD40-L |
Other Names | CD40-L, Tumor necrosis factor ligand superfamily member 5, TNF-related activation protein, TRAP, T cell antigen Gp39, CD154 antigen, sCD40, IGM, IMD3, HIGM1, T-BAM, TNFSF5, hCD40L. |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Results | 5-10 ng/ml |
Target/Specificity | CD40Ligand/TRAP |
Application Notes | Reconstitute in 5-10 mM Sodium Phosphate (pH 7.5) to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from 10 mM Sodium Phosphate, pH 7.5 |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD40 Ligand (CD40L/TRAP) is a type II membrane protein that effectuates the helper function of T cells on resting B cells. BioVision’s Human soluble CD40 Ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L
References
Graf D.,et al.Eur. J. Immunol. 22:3191-3194(1992).
Hollenbaugh D.,et al.EMBO J. 11:4313-4321(1992).
Aruffo A.,et al.Cell 72:291-300(1993).
Spriggs M.K.,et al.J. Exp. Med. 176:1543-1550(1992).
Gauchat J.-F.,et al.FEBS Lett. 315:259-266(1993).
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